These cytochromes also varied at the level of their charge at neutral pH and allowed to validate the importance of the electrostatic forces in the assembly of the biocomposite. For this reason, it has been substituted by other cytochromes, c3DvH, c7Da, c553DvH, c3DdN, and c3Dg, possessing lower redox potentials, in the range of 20 mV to -400 mV. NHE), was adapted to the oxidation of formate but was unsuitable for the reduction of fumarate. That indicates cytochrome c has, in addition to its structuring role, an electron shuttle activity. oneidensis, producing an oxidative current upon the addition of 1.5 mM glucose. Artificial biofilm was also tested with Pseudomonas fluorescens instead of S.
The current at the anode was 10 and 4 times higher with the artificial biofilm (0.027 A m2) than with film composed with bacteria alone (0.003 A m2) or associated with cytochrome c (0.007 A m2). Bacterial viability was studied and metabolic activity was characterized with the help of electrochemistry. These analyzes showed the importance of the cytochrome c in the assembly and organization of the biocomposite. The self-assembly was first studied by diffusion light scattering, scanning electron microscopy and Raman spectroscopy. A biocomposite material was proposed from the self-assembly of the bacteria Shewanella oneidensis with carbon nanotubes and cytochrome c (extract from bovine heart). The aim of this PhD work was to design an artificial electroactive biofilm in order to optimize extracellular electron transfers (EET) by artificially reconstituting the biofilm in the presence of exogenous materials.
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